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Labeled Staphylococcai Protein A as an Immunological Probe in the Analysis of Cell Surface Markers
Author(s) -
DORVAL G,
WELSH K. I.,
WIGZELL H
Publication year - 1974
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1974.tb01273.x
Subject(s) - antigen , cell , surface immunoglobulin , cluster of differentiation , antibody , population , biology , microbiology and biotechnology , lymphocyte , immunoglobulin g , chemistry , immunology , b cell , biochemistry , medicine , environmental health
Protein A from Staphylococcus aureus is known to interact in a specific manner with most subclasses of mammalian IgG, In our present work we have used protein A labeled with fluorescent or radioactive markers as a probe to detect cell‐associated IgG molecules. Using this approach to study human lymphocytes or lymphoid cell lines, we have found it possible to assess with accuracy the percentage of cells in a given population that carry a certain surface marker. Precise measurements can also be made as to quantities of cell surface‐attached IgG molecules, thereby allowing determinations of antigenic site numbers per cell in a new and simplified manner. We report here in detail the actual technical procedures involved, together with tests for human cell surface structures, including immunoglobulin molecules of IgG and other classes, HL‐A antigenic sites, beta2‐microglobulin, and T lymphocyte‐specific antigens