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Dynamic Aspects of the Interaction between Antibodies and Complement at the Cell Surface
Author(s) -
SUNDQVIST K G.,
SVEHAG S. E,
THORSTENSSON R T
Publication year - 1974
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1974.tb01254.x
Subject(s) - cytolysis , antibody , membrane , cell , cell membrane , chemistry , complement system , complement c1q , biophysics , biology , microbiology and biotechnology , biochemistry , immunology , in vitro , cytotoxicity
The possible influence of cell membrane dynamics on complement activation and cytolysis was studied. The interaction between membrane‐bound antibodies and complement was investigated by use of purified human C1q protein. The following observations were made: [1] Binding of C1q to cells was proportional to the amount of membrane‐bound antibody. [2] Demonstration of C1q at the cell surface was critically influenced by a prozone effect generated by the interaction between C1q and anti‐C1q antibody. [s] C1q induced pronounced patching of membrane‐bound antibody at 0°C and at 37°C, but its capping effect was weak. [4] C1q seemed to exert an inhibitory action on the disappearance of membrane‐bound antibody. The antibody‐bound C1q disappeared very rapidly from the cell membrane in comparison with the antibody. [5] The cytolytic sensitivity of several cell types differed at different stages of redistribution of membrane‐bound antibody; maximal sensitivity corresponded to a predominantly patchy antibody distribution. Microfluorometry and the isotope antiglobulin method suggested that these changes in cytolytic sensitivity was due neither to differences in the amount of membrane‐bound antibody nor to a different accessibility of membrane‐bound antibody to complement.