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Hapten‐Reactive T and B Mouse Lymphocytes
Author(s) -
SMITH E.,
HAMMARSTRÖM I.,
MÖLLER E.
Publication year - 1974
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1974.tb01233.x
Subject(s) - hapten , microbiology and biotechnology , antigen , antibody , population , spleen , antiserum , receptor , adjuvant , biology , chemistry , immunology , biochemistry , medicine , environmental health
The kinetics of development of anti‐hapten‐Kactive T and B cells in groups of mice immunized against different dotes of two hapten‐protein conjugates was measured at different times. after a primary immunization with a single batch of conjugated sheep erythrocytes. In addition, the affinity of the receptors on the specific antigen‐reactive cells was determined. Immunogenic doses of NNP‐HGG varied from 40 μ g to 2mg, whereas 0.4 μ g even with adjuvant were nonimmunogenic .The affinity of antibodies secreted by single cells was measured by a modification of the local hemolysis in‐gel assay with different concentrations of free hapten incorporated in the .agar. which showed a marked shift in affinity for i.PFC in contrast to d.PFC with time The affinityof receptor bearing antigen‐binding cells of both T and B origin was also Studied by a hapten inhibition assay With the aid of specific antisera against T and B spleen lymphoid cells. pure population of T or B cells could be obtained from spleens of mice immunizedwith different hapten‐protein conjugates .The binding strength of antigen‐binding B cells increased with time after immunization, whereas that of antigen‐binding T cells did not. The results are discussed in the light of recent studies on the structure of the T‐cell receptor