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Inhibition of Antibody‐Dependent Human Lymphocyte‐Mediated Cytotoxicity by Immunoglobulin Classes, IgG Subclasses, and IgG Fragments
Author(s) -
WISLØFF F.,
MICHAELSEN T. E.,
FRØLAND S. S.
Publication year - 1974
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1974.tb01230.x
Subject(s) - fragment crystallizable region , immunoglobulin d , antibody , immunoglobulin fc fragments , microbiology and biotechnology , chemistry , cytotoxicity , immunoglobulin g , immunoglobulin e , immunoglobulin heavy chain , fc receptor , receptor , biology , biochemistry , b cell , immunology , in vitro
The cytotoxicity of human peripheral blood lymphocytes against chicken erythrocytes sensitized by rabbit antibodies was inhibited by human immunoglobulin and immunoglobulin fragments. Myeloma proteins isolated in dimeric state or aggregated by heat treatment inhibited better than the corresponding monomeric proteins. Strong inhibition was observed with IgG1 and IgG3, and with IgG 2 after aggregation, while IgG 4 inhibited very little. No inhibition was found with IgM, IgA. IgD and IgE. The F(ab') 2 . and Fab fragments of IgG inhibited poorly or not at all. While‐ considerable inhibition was observed with the Fc fragment, the pFc’ fragment, which roughly corresponds to the C‐terminal half of the Fc portion, showed little inhibitory capacity. A fragment isolated from IgG3, containing an extension of the N‐terminal part of Fc (the Fch fragment), was an even better inhibitor than tin Fc fragment. The inhibitory capacity of the Fch and Fc fragments was greatly diminished following partial reduction and alkylation On the basis of the inhibitory pattern of IgG fragments, it is suggested that the region on the immunoglobulin molecule involved in binding to the Fc receptor of the effector lymphocytic cell may be located within the CH2 domain.