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23. Immunization, Isolation of Immunoglobulins, Estimation of Antibody Titre
Author(s) -
HARBOE N.,
INGILD AGNETE
Publication year - 1973
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1973.tb03798.x
Subject(s) - antibody , immunization , antiserum , titer , antigen , titration , virology , immunology , chemistry , chromatography , biology , inorganic chemistry
Summary. High titred monospecific antibodies are obtained in rabbits injecting as little as 25 μg pure antigen per kg body weight. For polyspecific antibodies the dose is increased 10 times. The immunization schedule is described in detail. A procedure is given for the isolation of the immunoglobulins from antiserum by salting out and ion exchange chromatography. Finally antibody titres as measured by 4 different titration methods are compared using anti‐human IgG from rabbits as a model.