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18. Determination of Insulin Binding to Human IgG by Quantitative Radioimmunoelectrophoresis
Author(s) -
CHRISTIANSEN AA. HEIN,
Krøll J.
Publication year - 1973
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1973.tb03793.x
Subject(s) - insulin , human insulin , chemistry , immunoglobulin g , antibody , immunoassay , medicine , endocrinology , chromatography , biology , immunology
Summary. The quantitative radioimmunoelectrophoresis for the determination of insulin binding to IgG is described. In 95% of 267 Danish diabetics treated with insulin, insulin‐binding IgG has been demonstrated. The method has the advantage of being specific, sensitive, and simple. A number of precautionary measures to be taken when using isotopes in electrophoresis are mentioned.