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Purification of Staphylococcal Protein A Using Immunosorbents
Author(s) -
KRONVALL GÖRAN
Publication year - 1973
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1973.tb02013.x
Subject(s) - staphylococcus aureus , protein a , elution , sepharose , chromatography , gamma globulin , chemistry , globulin , microbiology and biotechnology , antibody , biology , biochemistry , bacteria , enzyme , immunology , genetics
Protein A of Staphylococcus aureux is a marker probe for the Fe part of IgG‐1, −2 and −4 globulins and therefore of potential use in immunological work. A one‐step method for the rapid purification of protein A is described Staphylococcus aureus strains were grown in broth at 37deg;C and the protein A containing supernatants were mixed with immunosorbent containing protein A‐reactive normal human gammaglobulin. The excess broth was then washed away and protein A finally eluted off with 2 to 3 M KSCN. Using normal human gammaglobulin coupled to Sepharose 4 B the protein A preparations obtained were pure as judged by disc electrophoresis. Some previously described gel precipitation phenomena related to immunoglobulin reactivity with protein A, all occurred in a similar way using preparations of protein A produced by two different strains of Staphylococcus aureus .