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Separation of Cells According to Surface Antigens by the Use of Antibody‐Coated Columns. Fractionation of Cells Carrying Immunoglobulins and Blood Group Antigen
Author(s) -
WIGZELL H.,
SUNDQVIST K. G.,
YOSHIDA T. O.
Publication year - 1972
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1972.tb03737.x
Subject(s) - antibody , antigen , avidity , population , surface immunoglobulin , spleen , b 1 cell , chemistry , microbiology and biotechnology , immune system , biology , immunology , b cell , t cell , antigen presenting cell , medicine , environmental health
It has been found possible to separate cells according to their surface anti gens by the use of antibody‐coated columns. High efficiency columns were made by double‐layer principles, first coating beads with antigen followed by antibody in excess. Such columns could be shown to contain a high amount of free antigen‐binding sites for the relevant antigens. Lymphoid cells were thus fractionated according to their surface concentration of immunoglobu lin and a highly selective retention of mouse B lymphocytes was observed when filtering spleen cells through an anti‐immunoglobulin column prepared according to the above procedure. No evidence of retention of mouse T lymphoid cells was observed in the same system. By the use of anti‐gamma‐2a immunoglobulin columns, it was found possible to deplete a population from memory cells potentially capable of synthesizing gamma‐2a antibodies. No evidence was found that columns prepared in the described manner would function through combining with receptors on lymphoid cells for antibody‐antigen complexes. By using anti‐A blood group columns, it was possible to selectively retain cells (erythrocytes or kidney cells) with A blood group anti gen on their surface. High‐avidity immune antibodies were found to be more efficient than ‘natural’ anti‐A antibodies in this test. No evidence was found of anti‐A antibodies being adsorbed on to the passing cells as tested by in vitro serological tests and tissue culture experiments. The applications of a technique for separating cells according to their surface antigens are con sidered obvious.