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Formation of Hybrid IgM Subunits
Author(s) -
HARBOE M.,
SOLHEIM B. G.
Publication year - 1972
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1972.tb03736.x
Subject(s) - precipitin , antibody , monoclonal antibody , hemagglutination , antigen , immunoglobulin m , chemistry , microbiology and biotechnology , red cell , biology , biochemistry , immunology , immunoglobulin g , medicine
A mixture of rabbit IgM anti‐human A 1 red cell antibody and a human 125 I‐labelled monoclonal IgM was reduced with mercaptoethanot and exposed to oxygen under conditions in which most of the half subunits coalesced to IgM subunits with reformation of interchain disulphide bonds. Hybrid molecules were demonstrated in the mixture by precipitin tests with autoradiography and by binding of 125 l to human A 1 red cells. By careful selection of IgM antibodies with high energy in each binding site, such hybrids may be useful ‘IgM coats’ for haemagglutination inhibition tests attempting to define sub‐class‐specific and genetically determined antigens on IgM.