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Antigenic and Chemical Characterization of Non‐Immunoglobulin Amyloid Proteins
Author(s) -
HUSBY G.,
SLETTEN K.,
MICHAELSEN T. E.,
NATVIG J. B.
Publication year - 1972
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1972.tb03305.x
Subject(s) - sephadex , dithiothreitol , chemistry , antigen , antibody , guanidine , size exclusion chromatography , biochemistry , amyloid (mycology) , peptide , amino acid , microbiology and biotechnology , biology , immunology , enzyme , inorganic chemistry
The results of antigenic and chemical studies on subcomponents of amyloid fibrils secondary to chronic inflammatory diseases are reported. Treatment of amyloid with either guanidine and dithiothreitol or sodium hydroxide followed by gel filtration on Sephadex G‐100 revealed two major protein components, one eluted in the void volume (m. w. >200, 000), the other having an m. w. of 5, 000. Results from amino acid composition and N‐terminal sequences and also antigenic analysis suggest that the components are two different proteins not related to immunoglobulins.