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A Radial Diffusion Agar Plaque Technique for Estimation of the Avidity of Bactericidal Antibodies against Vibrio cholerae
Author(s) -
HOLMGREN J.,
SVENNERHOLM ANNMARI
Publication year - 1972
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1972.tb03300.x
Subject(s) - avidity , antiserum , microbiology and biotechnology , antigen , antibody , vibrio cholerae , chemistry , booster dose , toxoid , hapten , biology , immunization , immunology , bacteria , genetics
A radial diffusion agar plaque technique is presented for estimation of the average avidity of bactericidal antibodies, based upon quantitative inhibition of the antibodies with free lipopolysaccharide. With this technique and with Vibrio cholerae as model organisms the avidities of vibriocidal rabbit antisera were determined. It appeared that primary response antisera had lower avidity than secondary response antisera, which showed an inverse relation between avidity and booster antigen dose. However, the avidity differences observed in these systems were only 10‐ to 20‐fold, i. e. considerably less than reported for hapten‐protein conjugate antigen. Avidity estimations by the Fair ammonium‐sulphate precipitation method were in agreement with the results obtained with the described technique. The separated IgG and IgM fractions showed the described relation to immunization schedule; however, the IgM avidity appeared lower than the IgG avidity.