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Kinetics of the Reaction between a Monoclonal IgM Anti‐I and Rabbit Red Cells
Author(s) -
SOLHEIM B. G.
Publication year - 1972
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1972.tb00597.x
Subject(s) - chemistry , soybean agglutinin , monoclonal antibody , microbiology and biotechnology , kinetics , protein subunit , cold agglutinin , rabbit (cipher) , mole , agglutinin , biochemistry , antibody , lectin , biology , immunology , gene , physics , statistics , mathematics , quantum mechanics
Rabbit red cells were found to bind a maximum of 1.8 × 10 5 IgM anti‐I‐molecules. A K‐value of 5.0 ± 0.8 × 10 8 1/M was determined for the reaction between the cells and intact anti‐I at 5°C; the K‐value was little affected by changes in temperature. Hybrid 17S and 8S molecules composed of varying amounts of cold agglutinin and inactive IgM were prepared by reduction with 2‐mercaptoethanol and reassociation. The results obtained with the reassociated products indicate random reassociation at the half subunit level. It is concluded that a 17S fraction with four or more active half subunits shows binding similar to intact IgM, while 8S fractions with two active half subunits show binding similar to the cysteine‐reduced 8S IgM.