Premium
Spatiotemporal distribution of flavescence dorée phytoplasma in grapevine
Author(s) -
Prezelj N.,
Nikolić P.,
Gruden K.,
Ravnikar M.,
Dermastia M.
Publication year - 2013
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.2012.02693.x
Subject(s) - phytoplasma , biology , vine , botany , horticulture , titer , veterinary medicine , polymerase chain reaction , virology , restriction fragment length polymorphism , genetics , gene , medicine , virus
In this study quantitative real‐time PCR was used to follow the seasonal changes of flavescence dorée phytoplasma ( FD p) titre in grapevines of cv. Modra frankinja (syn. Blaufränkisch) and cv. Refošk (syn. Refosco'd'Istria) from two vineyards located in climatically different vine‐growing regions of Slovenia. Besides its known presence in the leaf veins, FD p was also detected in flowers, berry tissues and tendrils. In plants with high concentrations of FD p in tissues with symptoms, phytoplasma was also detected in symptomless tissues. A trend of decreasing FD p titre in all examined symptomless tissues from June to July and an increasing one throughout the growing season in tissues with symptoms was recorded. Accordingly, FD p was present in detectable amounts in flowers, petioles and veins of almost all infected plants in the late spring, and was detected in all examined tissue types in summer, with the highest titre in berries in August. The study showed that in the absence of plant health measurements an FD p infection may spread exponentially by a factor of 40 per year.