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Maize dwarf mosaic virus diversity in the Johnsongrass native reservoir and in maize: evidence of geographical, host and temporal differentiation
Author(s) -
Achon M. A.,
AlonsoDueñas N.,
Serrano L.
Publication year - 2011
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.2010.02364.x
Subject(s) - biology , sorghum , genetic diversity , population , agronomy , host (biology) , genetic variation , ecology , genetics , gene , demography , sociology
The genetic diversity and population structure of Maize dwarf mosaic virus (MDMV) was examined by analysis of the full coat protein gene of 539 isolates collected from maize and Johnsongrass ( Sorghum halepense ) from eight different maize‐growing areas in Spain. Restriction fragment length polymorphism analysis revealed that the MDMV population consisted of 49 genetic variants, with the three most frequent accounting for 44% of the isolates. This population was spatially structured according to the establishment of maize crops in the area. The highest nucleotide diversity values were observed in the old maize‐growing areas in the northeast of Spain (>0·211) and the lowest in the new maize‐growing areas in the west (<0·019). Moreover, the major genetic variants differed between the old and new maize‐growing areas. Evidence of host‐associated selection was found in the endemic area of the virus (Lleida), and aphid‐transmission studies suggested vector selection pressure. Assessment of the temporal evolution of the MDMV population in northeastern areas indicated that time was only significant in Lleida, where it explained 4·8% of the total variation over nine consecutive years.