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Genetic structure of the grapevine fungal pathogen Phaeomoniella chlamydospora in southeastern Australia and southern France
Author(s) -
Smetham G. M.,
Ades P. K.,
Péros J.P.,
Ford R.
Publication year - 2010
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.2010.02302.x
Subject(s) - biology , genetic diversity , genetic structure , haplotype , linkage disequilibrium , population , microsatellite , genotype , population genetics , genetic variation , sexual reproduction , genetics , allele , demography , gene , sociology
In this study, 18 microsatellite loci were used to examine the genetic structure and mode of reproduction of Phaeomoniella chlamydospora , the fungus that causes Petri disease of grapevine and is involved in another grapevine disease, esca. A total of 60 southeastern Australian isolates were tested and compared with 64 isolates from southern France. The French population possessed relatively high genotypic diversity ( G = 29·2) whilst the Australian population showed low genotypic diversity ( G = 11·1), consistent with a limited founder population. Haplotypes from four different grapevine cultivars were not significantly differentiated ( Φ pt values effectively zero). Likewise, genetic differentiation of haplotypes from different regions within each country was not significant, although small but significant genetic differentiation (9%) was identified between Australia and France. Based on bootstrapped cluster analysis, there did not appear to be any genetic groups within the overall sample of isolates. Significant linkage disequilibrium identified in both countries, together with overrepresented, widespread identical haplotypes, indicated limited genetic recombination and a largely clonal structure consistent with the absence of an observed sexual cycle in this species.