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First report of olive knot caused by Pseudomonas savastanoi pv. savastanoi in Nepal
Author(s) -
Balestra G. M.,
Lamichhane J. R.,
Kshetri M. B.,
Mazzaglia A.,
Varvaro L.
Publication year - 2009
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.2008.02007.x
Subject(s) - inoculation , biology , genbank , olea , horticulture , pseudomonas syringae , botany , agar , bacteria , gene , biochemistry , genetics
During 2006–2008, on olive plants (Olea europea) in Nepal, knots (0·5–3 cm in diameter) resembling those caused by Pseudomonas savastanoi pv. savastanoi were observed. Bacterial cells were isolated on nutrient agar medium and the isolates were positive for oxidase, pectinolytic and arginine dihydrolase activity, tobacco hypersensitivity; production of fluorescent pigments and of indole on KB and on MM media, respectively, and negative in the production of levan. Characteristic knot symptoms were observed on artificially inoculated plants within six months after inoculation. Molecular identification was achieved by sequencing the 16S rDNA region. This is the first record of olive knot disease on olive trees (Olea europea) in Nepal