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Occurrence and molecular characterization of Turkish isolates of Turnip mosaic virus
Author(s) -
Korkmaz S.,
Tomitaka Y.,
Onder S.,
Ohshima K.
Publication year - 2008
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.2008.01902.x
Subject(s) - turnip mosaic virus , brassicaceae , raphanus , biology , brassica , potyvirus , botany , plant virus , recombinant dna , crop , virology , virus , agronomy , genetics , gene
A total of 142 samples of plants showing symptoms of Turnip mosaic virus (TuMV) were collected from fields planted to Brassicaceae and non‐Brassicaceae crops in the southwest Marmora region of Turkey, during the 2004−06 growing seasons. Using enzyme‐linked immunosorbent assay (ELISA) TuMV was detected in the main brassica‐crop fields of Turkey, with an overall incidence of 13·4%. TuMV was detected in samples from Brussels sprouts, cabbage, wild mustard, radish and wild radish, but not cauliflower or broccoli. The full‐length sequences of the genomic RNAs of two biologically distinct isolates, TUR1 and TUR9, were determined. Recombination analyses showed that TUR1 was an intralineage recombinant, whereas TUR9 was a non‐recombinant. Phylogenetic analyses of the Turkish isolates with those from the rest of the world showed that the TUR1 and TUR9 isolates belonged to world‐ Brassica and Asian‐ Brassica / Raphanus groups, respectively. This study showed that TuMV is widely distributed in the Asia Minor region of Turkey.