Premium
Direct molecular evidence supports long‐spored microsclerotial isolates of Verticillium from crucifers being interspecific hybrids
Author(s) -
Clewes E.,
Edwards S. G.,
Barbara D. J.
Publication year - 2008
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.2008.01897.x
Subject(s) - biology , verticillium dahliae , ploidy , genetics , verticillium , interspecific hybrids , gene , hybrid , sequence analysis , interspecific competition , botany
There is no published evidence directly supporting the suggestion that long‐spored isolates of Verticillium dahliae are interspecific hybrids. Retention in the nuclear genome of long‐spored (amphihaploid) isolates of two sequence types where non‐hybrid short‐spored (haploid) isolates carry only single sequence types would provide such evidence. PCR amplicons for a partial β ‐tubulin gene and a 5 S rRNA‐associated sequence were cloned and sequenced to provide multiple sequences for individual isolates. For the 5 S rRNA sequence, two sequence types were found for long‐spored isolates, but only one for the haploid isolates. For the β ‐tubulin gene, two or three sequence types were found in long‐spored isolates, but only one in most haploid isolates. One haploid isolate gave two sequence types, one like that from the other haploid isolates and the second like the short‐sequence type from some long‐spored isolates. These results support the long‐spored isolates being interspecific hybrids between a ‘parental’ species similar to V. dahliae (haploid) and an unidentified second species. The presence of a sequence type in some long‐spored isolates similar to that in one haploid isolate suggests a third ‘parental’ species may have been involved, possibly via a short‐spored isolate that was itself hybrid.