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Genome‐wide identification of pathogenicity genes in Xanthomonas oryzae pv. oryzae by transposon mutagenesis
Author(s) -
Wang J. C.,
So B. H.,
Kim J. H.,
Park Y. J.,
Lee B. M.,
Kang H. W.
Publication year - 2008
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.2008.01884.x
Subject(s) - biology , transposon mutagenesis , transposable element , xanthomonas oryzae , genetics , xanthomonas oryzae pv. oryzae , mutant , gene , mutagenesis , sleeping beauty transposon system , xanthomonas
A transposon mutant library was constructed from the bacterial blight pathogen Xanthomonas oryzae pv . oryzae ( Xoo ) KACC10331 by Tn5 transposon mutagenesis. The susceptible rice cultivar Milyang 23 was inoculated with a total of 24 540 mutants resistant to kanamycin and 67 avirulent or reduced‐pathogenicity mutant strains were selected for study. Southern hybridization verified that 84 mutant strains had single‐copy insertions and their single‐transposon insertion sites were identified by sequencing analysis combined with thermal asymmetric interlaced (TAIL)‐PCR. The single‐transposon‐tagged sequences of 21 mutant strains belonged to pathogenicity‐related genes previously reported in Xanthomonas species, while the other 46 single‐transposon‐tagged sequences included diverse functional genes encoding, five cell‐wall‐degrading enzymes, three fimbrial and flagella assembly regulators, five regulatory proteins, 15 metabolic regulators and 18 hypothetical proteins, which were identified as novel pathogenicity genes of Xoo .