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Diversity and detection of Korean Erwinia pyrifoliae strains as determined by plasmid profiling, phylogenetic analysis and PCR
Author(s) -
Shrestha R.,
Lee S. H.,
Kim J. E.,
Wilson C.,
Choi S.G.,
Park D. H.,
Wang M. H.,
Hur J. H.,
Lim C. K.
Publication year - 2007
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.2007.01679.x
Subject(s) - biology , phylogenetic tree , genetics , plasmid , primer (cosmetics) , intergenic region , erwinia , genetic diversity , polymerase chain reaction , genomic dna , gene , genome , population , chemistry , demography , organic chemistry , sociology
Twenty‐five strains of Erwinia pyrifoliae were investigated for their plasmid profiles and genetic relatedness. Four types of plasmid profile were observed for the first time, suggesting intraspecific plasmid profile diversity in E. pyrifoliae . Moreover, BOX‐PCR and phylogenetic analysis based on the 16S‐23S intergenic transcribed spacer (ITS) region showed genetic variations among E. pyrifoliae strains, although all strains were clustered in one group and separated from E. amylovora . On the other hand, ERIC‐PCR and phylogenetic analysis based on partial groEL gene sequences revealed close genetic relatedness among the strains. Amplification with EpSPF and EpSPR primers of a fragment of approximately 0·65 kb from the genomic DNA of all E. pyrifoliae strains, but not from E. amylovora strains, suggested that this primer set is useful for identification of this pathogen.