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An oligonucleotide microarray‐based assay for identification of phytoplasma 16S ribosomal groups
Author(s) -
Nicolaisen M.,
Bertaccini A.
Publication year - 2007
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.2006.01558.x
Subject(s) - phytoplasma , biology , oligonucleotide , ribosomal rna , 16s ribosomal rna , microarray , microbiology and biotechnology , computational biology , oligomer restriction , polymerase chain reaction , genetics , gene , restriction fragment length polymorphism , gene expression
A method using consensus PCR followed by oligonucleotide microarray hybridization was developed for identification of phytoplasma 16Sr ribosomal groups. The array consisted of 21– to 33‐nt‐long oligonucleotides which were designed to hybridize to individual 16Sr groups. Two oligonucleotides were designed to detect all phytoplasma groups. The array could efficiently identify samples from 16SrI, 16SrII, 16SrIII, 16SrV, 16SrVI, 16SrVII, 16SrIX, 16SrX and 16SrXII ribosomal groups. This microarray‐based test represents a rapid method for detection of phytoplasmas in unknown samples and for identification of most 16Sr groups.