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Evidence for separate origins of the two Pseudomonas avellanae lineages
Author(s) -
Scortichini M.,
Natalini E.,
Marchesi U.
Publication year - 2006
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.2006.01352.x
Subject(s) - biology , gene flow , genetics , primer (cosmetics) , genetic diversity , insertion sequence , mantel test , gene , polymerase chain reaction , dna sequencing , genetic variation , evolutionary biology , genome , population , chemistry , demography , organic chemistry , transposable element , sociology
Pseudomonas avellanae is the causal agent of hazelnut ( Corylus avellana ) decline, both in northern Greece and central Italy, and two lineages related to the geographical origins of the pathogen have previously been identified. Forty strains, obtained from all the areas where the disease has so far been observed, and representing six different subpopulations of the two lineages, were further assessed using insertion‐sequence PCR genomic fingerprinting. The data previously obtained from repetitive‐sequence PCR using ERIC and BOX primer sets and insertion‐sequence PCR (IS50) were analysed using statistical methods, enabling genetic diversity and gene flow among the populations to be elucidated, as well as verifying the possible correlation between genetic diversity and geographical origin. The Mantel test performed with ERIC, BOX and IS50‐PCR data revealed that the P. avellanae populations that are spatially distant from each other are also genetically dissimilar: gene flow estimates confirmed this. The present study supports the hypothesis that P. avellanae originated separately in Greece and Italy, and that the two lineages of the pathogen underwent separate local evolution.