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Armillaria species on tea in Kenya identified using isozyme and DNA sequence comparisons
Author(s) -
Mwenje E.,
Wingfield B. D.,
Coetzee M. P. A.,
Nemato H.,
Wingfield M. J.
Publication year - 2006
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.2006.01347.x
Subject(s) - armillaria , armillaria mellea , biology , isozyme , botany , phylogenetic tree , specific identification , sequence analysis , restriction fragment length polymorphism , polymerase chain reaction , dna , genetics , enzyme , gene , biochemistry
The aim of this study was to identify seven Armillaria isolates obtained from diseased tea bushes in Kenya using pectic enzyme profiles, PCR‐RFLP and IGS‐I DNA sequence data. The combination of these identification methods confirmed the presence of three distinct Armillaria groups. One of these groups resembled Zimbabwean group I ( A. fuscipes ). The second group was phylogenetically closely related to A. mellea ssp. nipponica . The third group was different from all other African isolates examined, but had isozyme patterns, especially of pectin methylesterases (PMEs), similar to those of isolates related to A. mellea ssp. nipponica. Analyses of sequence data suggested that this group is phylogenetically closely related to A. hinnulea from Australia and New Zealand.