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Correlation of molecular markers and biological properties in Verticillium dahliae and the possible origins of some isolates
Author(s) -
Collins A.,
MercadoBlanco J.,
JiménezDíaz R. M.,
Olivares C.,
Clewes E.,
Barbara D. J.
Publication year - 2005
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.2005.01240.x
Subject(s) - verticillium dahliae , biology , amplicon , primer (cosmetics) , genetics , polymerase chain reaction , botany , gene , chemistry , organic chemistry
Haploid and amphihaploid Verticillium dahliae isolates were studied using PCR‐based molecular markers which: (i) discriminate the defoliating and nondefoliating pathotypes (two primer pairs INTD2f/r and INTND2f/r), and (ii) are species‐specific (primer pair 19/22). The results were compared with some known biological and other molecular properties of the isolates. Five discrete sequences of the 19/22 amplicon were found. Sequence 4 was associated with both defoliating isolates from Spain and nondefoliating isolates from Spain and USA; these pathotypes were separated by the primer pairs INTD2f/r and INTND2f/r, but the data showed that the primer espdef01 (derived from the 19/22 amplicon) cannot be used for this purpose. Amplicon sizes and sequences with primers 19/22 divided amphihaploid isolates from crucifers (thought to be interspecific hybrids) into those corresponding to the previously reported α and β groups. The β‐group isolates had either sequence 4 or 5 (these two differing by a single base). The distinct amplicon sequence 3 given by the α‐group isolates demonstrated that the V. dahliae ‐like ‘parent’ of this group was molecularly unlike any haploid isolate yet studied. The overall results are discussed in relation to phytosanitary considerations and the probability of defoliating or crucifer pathotypes arising de novo within Europe, either by selection or by interspecific hybridizations.