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Genetic characterization of Gremmeniella abietina var. abietina isolates from Spain
Author(s) -
Santamaría O.,
AlvesSantos F. M.,
Diez J. J.
Publication year - 2005
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.2005.01184.x
Subject(s) - biology , rapd , pinus <genus> , race (biology) , botany , phytosanitary certification , genetic variability , horticulture , genetics , genotype , gene , genetic diversity , demography , population , sociology
The taxonomic placement of Spanish isolates of Gremmeniella abietina var. abietina (Gaa) (race and biotype) was determined, for the first time, using RAPD markers and comparison of RAPD patterns for Spanish isolates and those originating from different regions of Europe and North America. Fifteen Spanish isolates of Gaa were isolated from recently dead shoots of 10 Pinus halepensis trees. Additionally, 17 isolates of Gaa from Canada, Finland, Switzerland and the USA, previously characterized according to race and biotype, were used. Five primers, previously reported to yield readable, reproducible and polymorphic fragments, were used in PCR reactions. The results showed that the Spanish isolates represent the European race and not the Alpine or northern biotypes. Spanish isolates appear to be clearly separated from all other biotypes within the EU race and preliminary data suggest that Spanish isolates have low genetic variability.