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Biological, serological and molecular characterization of a cucumber mosaic virus isolate from India
Author(s) -
HAQ Q. M. R.,
SINGH B. P.,
SRIVASTAVA K. M.
Publication year - 1996
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1996.tb02891.x
Subject(s) - biology , open reading frame , subgenomic mrna , cucumber mosaic virus , gene , virology , complementary dna , nucleic acid sequence , virus , plant virus , genome , amino acid , serology , peptide sequence , genetics , antibody
A. virus causing mosaic and leaf deformation of Physalis minima has been identified as an isolate of cucumber mosaic virus (CMV) on the basis of its transmission by aphids in a non‐persistent manner, polyhedral particles of 29 nm diameter, molecular weight of coat protein subunits us 24‐5 kDa. serological relationship with a CMV isolate and a tripartite single‐stranded RNA genome with a subgenomic RNA4‐ Furthermore. cDNA representing coat protein gene was synthesized and cloned. Complete nucleotide sequences (890 nt) were obtained which showed a coat protein gene open reading frame of 657 residues. THE nucleotide sequences provided the 218 amino ACID sequences of the coat protein. Nucleotide as well as amino acid sequences revealed more than 90% identity with the CMV subgroup I strains.