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Culture in vitro of Rhododendron and Erysiphe sp.
Author(s) -
KENYON D. M.,
DIXON G. R.,
HELFER S.
Publication year - 1995
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1995.tb02787.x
Subject(s) - biology , subculture (biology) , powdery mildew , pathogen , inoculation , tissue culture , conidium , spore , host (biology) , botany , in vitro , mildew , microbiology and biotechnology , plant tissue culture , horticulture , ecology , biochemistry
Successful dual cultures of a powdery mildew pathogen and Rhododendron ponticum plantlets were achieved using a tissue culture technique. Sporulation was observed within 1 week of inoculation. Comparative in vitro cultures were grown at 10, 15 and 20°C with host tissue transferred to fresh media at 2‐monthly intervals. Viable conidia capable of infecting host tissue in vivo were obtained from cultures maintained at 10 and 15°C after culture for more than 6 months. Isolates from three sources (Auchincruive, Benmore and Perth) were introduced into culture. Cultures inoculated with Perth and Benmore isolates exhibited increased host growth at 10 and 15°C, respectively compared with control plantlets. These effects may result from pathogen‐induced changes to host regulatory metabolism. The culture technique allows the long‐term maintenance of pathogen cultures under sterile conditions without requiring subculture.