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Genome characterization of a new strain of peanut chlorotic streak virus causing chlorotic vein banding disease of groundnut ( Arachis hypogaea L.) in India
Author(s) -
SATYANARAYANA T.,
BROGLIO E.P.,
SREENIVASULU P.,
REDDY D.V.R.,
NAYUDU M.V.
Publication year - 1995
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1995.tb02654.x
Subject(s) - biology , arachis hypogaea , vigna , virus , virology , genome , dna , southern blot , strain (injury) , capsid , molecular cloning , genetics , gene , botany , complementary dna , anatomy
The double‐stranded DNA of the chlorotic vein banding isolate of peanut chlorotic streak virus (PCISV‐CVB), isolated from purified virus, resolved into circular and linear molecules similar to those of other caulimoviruses. A physical map of viral DNA was constructed, which showed the PCISV‐CVB DNA to be circular and composed of approximately 8·2 kbp. A number of restriction sites were found to be shared with a similar caulimovirus, PCISV, Nevertheless, several differences between physical maps of the two viruses suggest that PCISV‐CVB should be considered as a distinct strain of PCISV. Bam HI‐cleaved PCISV‐CVB DNA was cloned into pUC 118 and was infectious when cleaved from the cloning vector and inoculated onto Vigna unguiculata.