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Characterization of chilli vein‐banding mottle virus isolated from pepper in Thailand
Author(s) -
SIRIWONG P.,
KITTIPAKORN K.,
IKEGAMI M.
Publication year - 1995
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1995.tb01696.x
Subject(s) - mottle , virus , biology , virology , myzus persicae , potato virus y , pepper , plant virus , potyvirus , tobacco mosaic virus , tobacco etch virus , horticulture , aphid
A virus with flexuous particles, previously designated chilli veinal mottle virus (CVMV)‐CM1, which causes dark green spot, green vein‐banding and leaf distortion on peppers in Thailand, was isolated and characterized. The host range of this virus was restricted to the Solanaceae. The mean particle length in crude sap was 765 nm and the mean particle width was 13 nm. The virus was purified from pepper or Nicotiana glutinosa by extraction in phosphate buffer, followed by chloroform clarification and precipitation with PEG. The partially purified virus was further purified by sucrose density gradient centrifugation. This protocol consistently yielded 8–19mg virus/kg infected leaves. The purified virus had a UV absorption spectrum typical of nucleoprotein, with a minimum at 246 nm, a maximum at 260 nm, and a slight shoulder at 290 nm. The A 260 /A 280 ratio was in the range 1·20–1·28, and the A max /A min ratio was in the range 1·11–1·27; the buoyant density in CsCl was 1·328g/cm 3 Virions contained a single‐stranded RNA of c. 10kb and a single major protein of molecular weight 34000. The virus induced cytoplasmic inclusions in infected cells, which were observed as pinwheels, scrolls and laminated aggregates. It was not serologically related to potato virus Y (PVY, PVY‐O, PVY‐T), pepper mottle virus (PepMoV), pepper veinal mottle virus (PVMV), tobacco etch virus (TEV), papaya ringspot virus (PRSV), or peanut stripe virus (PStV), but is related to chilli veinal mottle virus occurring in Malaysia. On the basis of particle morphology, physico‐chemical and biological properties and cytopathology, it is considered to be a potyvirus. As this virus was serologically distinct from well‐known potyviruses, it is thought to be a new pepper potyvirus found in Thailand. Since the virus induces a typical dark green spotting, patching and banding along the vein in established infected peppers, the new and more descriptive name ‘chilli vein‐banding mottle virus’(CVbMV) is proposed.