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Essential oil production increased by using virus‐free patchouli plants derived from meristem‐tip culture
Author(s) -
SUGIMURA Y.,
PADAYHAG B. F.,
CENIZA M. S.,
KAMATA N.,
EGUCHI S.,
NATSUAKI T.,
OKUDA S.
Publication year - 1995
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1995.tb01673.x
Subject(s) - patchouli , pogostemon , biology , cutting , meristem , shoot , botany , virus , horticulture , tissue culture , essential oil , biomass (ecology) , agronomy , in vitro , virology , biochemistry , traditional medicine , medicine
For the production of virus‐free patchouli ( Pogostemon cablin ), isolated meristem tips were cultured on a medium supplemented with 0·2 ppm 6‐benzylaminopurine. Multiple shoot proliferation was initiated during the culture. Complete plantlets were regenerated by transferring multiple shoots to a medium devoid of phytohormones. Plantlets thus produced were transplanted to soil with a high rate of survival and tested by enzyme‐linked immunosorbent assay (ELISA) to check elimination of patchouli mild mosaic virus. Following further propagation by stem cuttings, virus‐free plants were grown in the northern and southern parts of Mindanao Island, Philippines. Significant increases in biomass and essential oil yield were observed in virus‐free plants grown at both localities. Using gas chromatographic analyses, a full set of major sesquiterpenes was detected in the essential oils recovered from virus‐free patchouli plants. Reinfection of healthy clones with the virus took place within 4 months of cultivation in the field.