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Detection of the source of infection of apple trees by Cylindrocarpon heteronema using DNA polymorphisms
Author(s) -
BROWN A. E.,
MUTHUMEENAKSHI S.,
SWINBURNE T. R.,
LI R.
Publication year - 1994
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1994.tb02693.x
Subject(s) - biology , restriction fragment length polymorphism , mitochondrial dna , ribosomal dna , genetics , intraspecific competition , dna , ribosomal rna , spacer dna , internal transcribed spacer , polymerase chain reaction , microbiology and biotechnology , gene , zoology , phylogenetics
Restriction fragment length polymorphisms (RFLPs) of the ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) of isolates of Cylindrocarpon heteronema were analysed using rDNA from Saccharomyces carlsbergensis and mtDNA from C. heteronema as probes. These analyses revealed intraspecific heterogeneity; four rDNA and six mtDNA restriction pattern categories were observed among the isolates tested. The two main rDNA RFLP categories, both of which subdivided into two mtDNA categories, detected within the UK isolates could not be associated with the localities from which they were obtained, but the majority of isolates originating from trees produced in the same nursery, irrespective of where they were finally planted, belonged to the same category.