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Double‐stranded RNA comprising the putative avocado virus 1 has a high degree of sequence homology to the avocado genome
Author(s) -
COOK G.,
KOTZÉ J. M.,
NEL L. H.
Publication year - 1994
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1994.tb01636.x
Subject(s) - biology , rna silencing , rna , complementary dna , dna , genome , virology , homology (biology) , plant virus , genetics , nucleic acid sequence , virus , gene , rna interference
The hypothesis that the double‐stranded (ds) RNA isolated from avocado is indicative of infection by RNA plant viruses has never been proven. However, it has not been disproven and the different dsRNAs are still known as putative avocado viruses (AV) 1, 2 and 3. We have probed restriction fragments of avocado cellular DNA with cloned 32 P‐labelled AV1‐specific cDNA and discovered that the dsRNA nucleotide sequences are also present on plant DNA. Our evidence contradicts the hypothesis that viruses are responsible for the formation of the dsRNA species in avocado.