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Maintenance of mycoplasma‐like organisms occurring in Pyrus species by micropropagation and their elimination by tetracycline therapy
Author(s) -
VIES D. L.,
CLARK M. F.
Publication year - 1994
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1994.tb01626.x
Subject(s) - biology , micropropagation , tetracycline , pear , botany , mycoplasma , microbiology and biotechnology , in vitro , antibiotics , tissue culture , genetics
Pear explants ( Pyrus communis cv. Pine) infected with mycoplasma‐like organisms (MLOs) were maintained for more than 3 years by micropropagation on Murashige and Skoog medium supplemented with gibberellic acid, indole butyric acid and benzyl amino purine. MLOs reached consistently higher concentrations in micropropagated explants than in samples from field‐grown plants, although explants remained free from symptoms. MLOs could be eliminated by incorporation of 100/μg/ml oxytetracycline into the growth medium for a period of 4 weeks. These results have implications for plant propagation schemes of symptomless MLO‐infected explants raised by micropropagation.