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A third cryptic virus in beet ( Beta vulgaris )
Author(s) -
XIE W. S.,
ANTONIW J. F.,
WHITE R. F.,
WOODS R. D.
Publication year - 1993
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1993.tb01524.x
Subject(s) - biology , rna silencing , complementary dna , virus , plant virus , rna , microbiology and biotechnology , virology , homology (biology) , southern blot , dna , genetics , gene , rna interference
Japanese leaf beet Beta vulgaris var. cicla cv. Fudanso plants were found to contain four double‐stranded RNA (dsRNA) components in apparently healthy beet plants. Two were identified as from beet cryptic virus 1 (BCV1), but the other two showed different mobilities on gel electrophoresis and were transcribed into complementary DNA (cDNA) and cloned. Hybridization analysis showed no significant sequence homology between these two dsRNAs and the dsRNA components of BCV1 or the other known cryptic virus of beet, BCV2. Slot‐ and dot‐blot hybridization were used with cDNA clones as probes to identify plants containing these two dsRNA components. Virus particles were purified from these plants and were shown to contain the two new dsRNA components, thus demonstrating the existence of a new beet cryptic virus, which we have called BCV3.