Premium
Hypersensitive reaction in alfalfa ( Medicago sativa ) induced by alfalfa mosaic virus
Author(s) -
ESBROECK Z. PESICVAN,
HIRUKI C.
Publication year - 1990
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1990.tb02529.x
Subject(s) - medicago sativa , inoculation , biology , virology , alfalfa mosaic virus , virus , hypersensitive response , plant virus , botany , horticulture , plant disease resistance , rna , coat protein , biochemistry , gene
Alfalfa (Medicago sativa) cv. Beaver was screened for resistance to alfalfa mosaic virus (AIMV). A1MV infection in clonal plants was tested by double‐antibody sandwich ELISA 4 and 8 weeks after inoculation with purified A1MV (1 mg/ml in 0025 M phosphate buffer, pH 7 0). Twelve clones showed a hypersensitive reaction 3–4 days after inoculation and the infection was restricted to the inoculated leaves. All the plants of hypersensitive clones consistently produced local lesions when inoculated with purified AIM V. In contrast, plants inoculated with AIMV in crude sap remained symptomless, although AIMV was detectable in inoculated leaves. The remaining 16 clones were susceptible to AIMV and showed systemic infection.