Premium
Serological and molecular evidence for the complexity of the leafroll disease of grapevine
Author(s) -
TANNE EDNA,
NAVEH LEA,
SELA ILAN
Publication year - 1989
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1989.tb02132.x
Subject(s) - antiserum , biology , virology , potyvirus , dot blot , serology , western blot , virus , vitis vinifera , southern blot , microbiology and biotechnology , antibody , plant virus , gene , horticulture , genetics
Samples of leafroll‐infected grapevines from various countries, as well as local sources, were tested by ELISA with antisera against two closteroviruses (GVA and NY‐1) and a potyvirus (GPV) thought to be associated with leafroll. Many of the samples reacted positively with more than one antiserum, and often with all three. Positive reactions with GPV antiserum were corroborated by molecular dot‐blot hybridization. One of the closteroviruses, grapevine virus A (GVA) was originally isolated from a grapevine affected by stem‐pitting; furthermore, a grapevine with stem‐pitting reacted positively with all three antisera, and with a potyvirus probe by dot‐blot hybridization. All the leafroll‐diseased vines reacted, at least in some tests, with one or more of the antisera. Hence, in spite of the complexity of this syndrome, healthy and diseased grapevines could be distinguished.