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Effect of potato physiology on the interpretation of ELISA results for potato leafroll virus
Author(s) -
GUNN L. V.,
PARES R. D.
Publication year - 1988
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1988.tb02109.x
Subject(s) - potato leafroll virus , biology , antiserum , virology , virus , antigen , serology , host (biology) , plant virus , antibody , immunology , ecology
This paper records the occurrence of an antigen produced in plants infected by potato leafroll virus (PLRV), which is copurified with the virus and induces an immune response in rabbits used for virus antiserum production. The antigen also appeared to be produced in uninfected but physiologically stressed potato plants. These plants reacted with antisera to PLRV in ELISA tests, thereby giving false positive results. We refer to the compound as virus‐stress antigen (VSA). The importance of this finding, not only to serological testing for PLRV, but also as a possible explanation for some false‐positive reactions that occur with other host‐virus combinations, is discussed. The necessity of having detailed information on plants from which samples are taken for testing is emphasized.