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A new procedure to detect a low incidence of Ustilago nuda in seed barley
Author(s) -
HEWETT P. D.,
DAMGACI E.
Publication year - 1986
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1986.tb02029.x
Subject(s) - ustilago , mycelium , biology , botany , filter paper , digestion (alchemy) , horticulture , chromatography , biochemistry , gene , chemistry
Partial enzymic digestion of extracted barley embryos enabled the mycelium of Ustilago nuda to be separated and collected on a nylon mesh filter. Mycelium from samples with less than 0.1% seeds infected was readily detectable. The procedure promises to be a new and sensitive routine method.