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Serological detection of Fiji disease virus antigens in the planthopper Perkinsiella saccharicida and its inefficient ability to transmit the virus
Author(s) -
FRANCKI R. I. B.,
RYAN C. C.,
HATTA T.,
ROHOZINSKI J.,
GRIVELL C. J.
Publication year - 1986
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1986.tb02022.x
Subject(s) - immunoelectron microscopy , biology , virology , virus , antigen , serology , transmission (telecommunications) , antibody , immunology , electrical engineering , engineering
Individual adult Perkinsiella saccharicida reared on sugarcane infected with Fiji disease virus (FDV) were confined on healthy plants for 7 days to test for virus transmission and were then sacrificed and assayed for the presence of FDV‐specific structures by immunoelectron microscopy. Only 15% of the insects contained FDV antigens and 6% transmitted the virus. FDV‐specific antigens were detected in individual planthoppers by immuno‐osmophoretic and enzyme‐linked immunosorbent assays about as efficiently as by immunoelectron microscopy. The possible reasons for the inefficient acquisition and transmission of FDV by P. saccharicida are discussed.