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Enzyme‐linked immunosorbent assays for study of serological relationships and detection of three luteoviruses
Author(s) -
D'ARCY CLEORA J.,
HEWINGS ADRIANNA D.
Publication year - 1986
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1986.tb02017.x
Subject(s) - biology , luteovirus , serology , virology , heterologous , strain (injury) , virus , barley yellow dwarf , dwarfing , plant virus , antibody , veterinary medicine , gene , genetics , medicine , anatomy
Enzyme‐linked immunosorbent assay (ELISA) systems were used to examine serological relationships and to detect three luteoviruses; a vector‐non‐specific strain of barley yellow dwarf virus from Illinois (BYDV‐PAV‐IL), a strain of beet western yellows virus from California (BWYV‐CA) and a dwarfing strain of soybean dwarf virus from Japan (SDV‐D). Indirect ELISA (IND‐ELISA) systems detected distant reciprocal serological relationships among all three viruses. This is the first report of a serological relationship between a vector‐non‐specific strain of BYDV and any strain of SDV. Double antibody sandwich ELISA (DAS‐ELISA) systems detected the purified homologous viruses at concentrations as low as 1.6 ng/ml. hut did not detect heterologous viruses as concentrated as 800 ng/ml. In contrast, when DAS‐ELISA systems were used for detection of the three viruses in sap extracts from infected plants some weak but significant (P=0.05 ) heterologous reactions occurred. The BYDV‐PAV‐IL DAS‐ELISA system usually detected BWYV‐CA and sometimes detected SDV‐D; the BWYV‐CA DAS‐ELISA system never detected BYDV‐PAV‐IL and rarely detected SDV‐D; the SDV‐D DAS‐ELISA system sometimes detected BYDV‐PAV‐IL and consistently detected BWYV‐CA.