Premium
Some properties of a virus‐like agent found in Brachypodium sylvaticum in the United Kingdom
Author(s) -
EDWARDS M.–L.,
COOPER J. I.,
MASSALSKI P. R.,
GREEN B.
Publication year - 1985
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1985.tb02765.x
Subject(s) - biology , botany , inoculation , infectivity , dilution , sucrose gradient , particle (ecology) , virus , ultracentrifuge , sedimentation coefficient , horticulture , biochemistry , virology , enzyme , ecology , physics , thermodynamics
A spherical virus–like agent ($$32 nm in diameter) was isolated from a plant of Brachypodium sylvaticum (Huds.) Beauv. growing in a botanic garden in England and showing yellow streaks in the leaves. The agent was readily purified and sedimented as a single component in sucrose density rate gradients. The particles had a sedimentation coefficient at infinite dilution of $$122S and a buoyant density of 1.35 g/cm 3 in CsCl and 1·33 in CS 2 SO 4 . The particles were stable at acid pH but above pH 7·0 in the presence of EDTA dissociated. A protein having a major polypeptide with a molecular weight of $$3·76 × 10 4 and a species of single stranded RN A with a MW of 1·67 × 10 6 were detected in the particles. The agent was not transmitted by manual inoculation, by the insects Myzus persicae Sulzer, Rhopalosiphum padi L. or Nephotettix virescens Distant, through soil by leakage from roots or by seed. The particles had physicochemical properties in common with tombus– and sobemoviruses but were not serologically related to 10 members of these groups or to 57 other small spherical RNA plant viruses.