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Methods for visualizing hyaline fungi for the detection of antifungal compounds on thin‐layer chromatograms
Author(s) -
WOODWARD S.,
PEARCE R. B.
Publication year - 1985
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1985.tb01396.x
Subject(s) - heterobasidion annosum , mycelium , biology , fungus , thin layer chromatography , bark (sound) , microbiology and biotechnology , fluorescein , substrate (aquarium) , hypha , hyaline , botany , thin layer , esterase , chromatography , fluorescence , biochemistry , layer (electronics) , picea abies , chemistry , enzyme , organic chemistry , ecology , physics , quantum mechanics
Two methods facilitating the use of hyaline fungi for the detection of antifungal compounds on thin‐layer chromatograms are described. Fungal growth was visualized under ultraviolet light either by the metabolic release of fluorescein from the esterase substrate fluorescein diacetate, or by the binding of a fluorescent brightener (Calcofluor White M2R New, or Tinopal‐CBS) to the mycelium on the TLC plate. These methods were used to demonstrate compounds inhibitory to the wood‐decay fungus Heterobasidion annosum in bark extracts of Sitka spruce ( Picea sitchensis ).