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Biochemical characteristics of sporelings of Puccinia graminis f. sp. tritici and protoplasts from wheat isolines of potential significance in specificity
Author(s) -
HOLDEN D. W.,
STRANGE R. N.
Publication year - 1984
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1984.tb02645.x
Subject(s) - biology , wheat germ agglutinin , puccinia , protoplast , appressorium , germ tube , concanavalin a , microbiology and biotechnology , poaceae , hypha , lysis , botany , neurospora crassa , agglutinin , lectin , hordeum vulgare , biochemistry , gene , mutant , mildew , in vitro
The affinity of various lectins for protoplasts from isolines of wheat differing in their reaction to Puccinia graminis Pers. f. sp. tritici , Ericks and E. Henn. race 21 and for infection structures of the fungus was studied. Protoplasts of both isolines were agglutinated by concanavalin A and soybean lectin and lysed by wheat germ agglutinin. Pokeweed mitogen and wheat germ agglutinin bound to fungal germ tubes and appressoria but not to uredospore walls, substomatal vesicles or infection hyphae. Viability of protoplasts from either isoline was not affected when they were incubated with uredospores, germlings with and without infection structures or with an extract of the susceptible line, heavily infected by the fungus. No differences between polypeptide fractions prepared from protoplasts of the isolines were detected by sodium dodecyl sulphate polyacrylamide gel electrophoresis.