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The use of a tomato cotyledon test to identify Corynebacterium michiganense pv. michiganense
Author(s) -
CHALDECOTT M. A.,
PREECE T. F.
Publication year - 1983
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1983.tb02859.x
Subject(s) - cotyledon , biology , inoculation , lycopersicon , corynebacterium , bacteria , horticulture , botany , microbiology and biotechnology , genetics
When cotyledons of 4‐day‐old tomato seedlings (Lycopersicon esculentum cv. Sonato) were inoculated with Corynebacterium michiganense pv. michiganense and kept at 25°C with a 16 h light period per day, white raised blisters were produced which coalesced to give white, scurfy, cracked areas after 3 days. This symptom did not develop when Gram‐positive coryneform bacteria isolated from, and near, tomato crops were used as inoculum. Pre‐inoculation abrasion of cotyledons, an inoculum concentration of at least 10 5 bacteria/ml, and high humidity after inoculation were important factors in the expression of this host reaction. The cotyledon test may be of value in laboratories where more detailed tests for C. michiganense pv. michiganense are not possible.