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Rearing Tipula oleracea (Diptera, Tipulidae) in Laboratory Cultures
Author(s) -
CARTER J. B.
Publication year - 1975
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1975.tb01871.x
Subject(s) - biology , larva , brassica oleracea , agar , botany , horticulture , agar plate , bacteria , genetics
SUMMARY Tipula oleracea L. was cultured in the laboratory at 20°C, producing five to six generations per year and, for example, 20,000 surplus insects for experimental purposes. The eggs were kid in agar and the larvae were reared in sand and Fed dried, powdered grass. Small insects such as sciarids contaminated the culture but were controlled by mechanical separation and heat treatment of the sand. This method has been used to rear T. oleracea through 35 generations.