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A Micro‐container Technique for Studying Virus Transmission by Nematodes *
Author(s) -
VALDEz R. B.
Publication year - 1972
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1972.tb01739.x
Subject(s) - biology , inoculation , transmission (telecommunications) , horticulture , container (type theory) , petunia , virus , botany , veterinary medicine , virology , mechanical engineering , medicine , biochemistry , gene , electrical engineering , engineering
SUMMARY Previous methods for studying virus transmission by nematodes were improved to achieve up to 100 per cent infection when using single specimens of Xiphinema diversicaudatum (Micol.) and small petunia test plants grown in micro‐containers kept inside moist chambers. Four ml of steamed soil/sand (2:1) mixture were used during test feeding, and more was added as the plants grew. The roots were tested for infection by inoculating appropriate indicator plants and the virus was identified serologically.