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Tobacco Streak Virus in Dahlias
Author(s) -
Brunt A. A.
Publication year - 1968
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1968.tb00433.x
Subject(s) - biology , nicotiana tabacum , tobacco mosaic virus , antiserum , inoculation , chenopodium , virus , virology , cultivar , chenopodium quinoa , titer , plant virus , horticulture , botany , antibody , biochemistry , gene , immunology , weed
SUMMARY Tobacco streak (TSV), a virus hitherto unrecorded in Britain, was isolated from five of 73 cultivars in three of six stocks of dahlias tested. Three cultivars infected with TSV alone were symptomless, and 12 dahlia seedlings (‘Ideal Bedder’) infected by mechanical inoculation produced only inconspicuous leaf symptoms within six months. TSV is unstable in vitro but was isolated from dahlias without difficulty when inocula were prepared with cold phosphate buffer containing either thioglycolhc acid or sodium diethyldithiocarbamate. The virus was readily distinguished from cucumber mosaic, dahlia mosaic and tomato spotted wilt viruses by the symptoms it induced in Chenopodium amaranticolor, C. murale, C. quinoa and Nicotiana tabacum . Partially purified virus preparations reacted in serological gel‐diffusion tests with an antiserum to a North American isolate of TSV but not with antisera to 23 isometric viruses commonly occurring in Britain. Like those of other well‐characterised isolates, purified preparation of TSV from dahlias were infective, contained numerous ‘spherical’ particles c . 30mû in diameter, contained two components with sedimentation coefficients of 85 and 98 S and produced two zones when centrifuged in sucrose density‐gradient columns.