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A role for oxalic acid generation in ozone‐induced signallization in Arabidopis cells
Author(s) -
TRAN DANIEL,
KADONO TAKASHI,
MOLAS MARIA LIA,
ERRAKHI RAFIK,
BRIAND JOËL,
BILIGUI BERNADETTE,
KAWANO TOMONORI,
BOUTEAU FRANÇOIS
Publication year - 2013
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.2012.02596.x
Subject(s) - oxalic acid , reactive oxygen species , arabidopsis thaliana , cytosol , programmed cell death , chemistry , ozone , depolarization , apoptosis , plant cell , biochemistry , microbiology and biotechnology , biophysics , biology , organic chemistry , gene , mutant , enzyme
Ozone (O 3 ) is an air pollutant with an impact increasingly important in our industrialized world. It affects human health and productivity in various crops. We provide the evidences that treatment of Arabidopsis thaliana with O 3 results in ascorbate‐derived oxalic acid production. Using cultured cells of A. thaliana as a model, here we further showed that oxalic acid induces activation of anion channels that trigger depolarization of the cell, increase in cytosolic Ca 2+ concentration, generation of reactive oxygen species and cell death. We confirmed that O 3 reacts with ascorbate in the culture, thus resulting in production of oxalic acid and this could be part of the O 3 ‐induced signalling pathways that trigger programmed cell death.