Premium
Identification of OsbHLH133 as a regulator of iron distribution between roots and shoots in Oryza sativa
Author(s) -
WANG LU,
YING YINGHUI,
NARSAI REENA,
YE LINGXIAO,
ZHENG LUQING,
TIAN JINGLUAN,
WHELAN JAMES,
SHOU HUIXIA
Publication year - 2013
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.2012.02569.x
Subject(s) - oryza sativa , shoot , xylem , mutant , botany , biofortification , regulator , biology , wild type , rice plant , micronutrient , plant growth , chemistry , biochemistry , microbiology and biotechnology , gene , horticulture , organic chemistry
Iron (Fe) is an essential micronutrient element for plant growth. Regulation of Fe‐deficiency signalling networks is one of the many functions reported for basic helix‐loop‐helix (bHLH) transcription factors in plants. In the present study, OsbHLH133 was found to be induced by Fe‐deficiency conditions in Oryza sativa . Insertional inactivation of OsbHLH133 ( bhlh133 ) resulted in growth retardation, with enhanced Fe concentration seen in shoots, and reduced Fe concentration in roots. Overexpression of OsbHLH133 had the opposite effect, that is resulted in an enhanced Fe concentration in roots and reduced Fe concentration in shoots and also in xylem sap. Microarray analysis showed that some of the genes encoding Fe‐related functions were up‐regulated under Fe‐sufficient conditions, in bhlh133 mutant plants compared to wild‐type plants. Significant differential expression of a number of signalling pathways, including calcium signalling, was also seen in bhlh133 plants compared to wild‐type plants, independent of Fe conditions.