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Nitric oxide production mediates oligogalacturonide‐triggered immunity and resistance to Botrytis cinerea in Arabidopsis thaliana
Author(s) -
RASUL S.,
DUBREUILMAURIZI C.,
LAMOTTE O.,
KOEN E.,
POINSSOT B.,
ALCARAZ G.,
WENDEHENNE D.,
JEANDROZ S.
Publication year - 2012
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.2012.02505.x
Subject(s) - botrytis cinerea , arabidopsis thaliana , nitric oxide , nitrate reductase , reactive oxygen species , peroxidase , arabidopsis , mutant , biology , plant defense against herbivory , scavenger , callose , microbiology and biotechnology , botany , biochemistry , chemistry , enzyme , cell wall , gene , antioxidant , endocrinology
Nitric oxide (NO) regulates a wide range of plant processes from development to environmental adaptation. In this study, we investigated the production and/or function of NO in Arabidopsis thaliana leaf discs and plants elicited by oligogalacturonides (OGs) and challenged with Botrytis cinerea . We provided evidence that OGs triggered a fast and long lasting NO production which was Ca 2+ dependent and involved nitrate reductase (NR). Accordingly, OGs triggered an increase of both NR activity and transcript accumulation. NO production was also sensitive to the mammalian NO synthase inhibitor L‐NAME. Intriguingly, we showed that L‐NAME affected NO production by interfering with NR activity, thus questioning the mechanisms of how this compound impairs NO synthesis in plants. We further demonstrated that NO modulates RBOHD‐mediated reactive oxygen species (ROS) production and participates in the regulation of OG‐responsive genes such as anionic peroxidase (PER4) and a β ‐1,3‐glucanase. Mutant plants impaired in PER4 and β ‐1,3‐glucanase, as well as Col‐0 plants treated with the NO scavenger cPTIO, were more susceptible to B. cinerea. Taken together, our investigation deciphers part of the mechanisms linking NO production, NO‐induced effects and basal resistance to B. cinerea .